u6 promoter in sgopti49 (Addgene inc)
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u6 promoter in sgopti49
U6 Promoter In Sgopti49, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/u6+promoter+in+sgopti49/sgOpti+(Plasmid+%2385681)/pm41832176-299-36-40
Average 93 stars, based on 51 article reviews
U6 Promoter In Sgopti49, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/u6+promoter+in+sgopti49/sgOpti+(Plasmid+%2385681)/pm41832176-299-36-40
Average 93 stars, based on 51 article reviews
u6 promoter in sgopti49 - by Bioz Stars,
2026-09
93/100 stars
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Plasmid Preparation:Article Title: Cell type-specific enhancers regulate IL-22 expression in innate and adaptive type 3 lymphoid cells. Article Snippet: Next, TRE3G promoter and pHR-pEFEGFP vector47 (Addgene Plasmid #67952) were digested with EcoR1+BamH1 and ligated, resulting in pHR-TRE3G-EGFP vector. .. Lastly, dCas9-KRAB-P2A-mcherry cassette was swapped from pHR-SFFVKRAB-dCas9-P2A-mCherry48 (Addgene Plasmid #60954) into pHR-TRE3G-EGFP vector from above using Mlu1 and Not1 restriction sites. sgRNAs targeting Il22 promoter, E22-1, E22-2, twin-E22-2, or scrambled sgRNA were cloned downstream of the Clone Assay:Article Title: Cell type-specific enhancers regulate IL-22 expression in innate and adaptive type 3 lymphoid cells. Article Snippet: Next, TRE3G promoter and pHR-pEFEGFP vector47 (Addgene Plasmid #67952) were digested with EcoR1+BamH1 and ligated, resulting in pHR-TRE3G-EGFP vector. .. Lastly, dCas9-KRAB-P2A-mcherry cassette was swapped from pHR-SFFVKRAB-dCas9-P2A-mCherry48 (Addgene Plasmid #60954) into pHR-TRE3G-EGFP vector from above using Mlu1 and Not1 restriction sites. sgRNAs targeting Il22 promoter, E22-1, E22-2, twin-E22-2, or scrambled sgRNA were cloned downstream of the |